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US 12,638,438 B2Granted
Screening methods using canine T2R receptors and pet food products and compositions identified using the same
AssigneeMars, Incorporated
InventorsMatthew Ronald Gibbs, Neil George Desforges, Andrew John Taylor, Scott Joseph McGrane, Boris Klebansky, Richard Masten Fine
Life of this patent
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Abstract
The presently disclosed subject matter relates to methods of screening raw materials and pet food products to manufacture a palatable pet food product. The presently disclosed subject matter also relates to methods for identifying compounds that modulate the activity and/or expression of a bitter taste receptor.
Claims11
A method for producing a pet food product, the method comprising:
- (a) measuring a first intracellular calcium level of a cell expressing a bitter taste receptor;
- (b) contacting a test compound with the cell;
- (c) measuring a second intracellular calcium level of the cell;
- (d) selecting the test compound as capable of reducing the bitter taste when the second intracellular calcium level is lesser than the first intracellular calcium level;
- and
- (e) admixing the test compound with a pet food product to thereby reduce bitter taste;
- wherein the bitter taste receptor is a T2R2 comprising the amino acid sequence set forth in SEQ ID NO:
- 18.
5 more elements
Dependencies are inferred from the claim text.