Spike-in reference standard for use in detecting sample target from DNA or RNA organism

A mock virus vector, wherein a viral backbone is used as the vector;

• the viral backbone contains one or more quantitative detection nucleic acid fragments and the coding gene of a fluorescent protein for tracking,
• the quantitative detection nucleic acid fragments has the same length as the nucleic acid target sequence of a test sample, and has the same percentage of base composition as the nucleic acid target sequence of the test sample;
• wherein the 5′ end sequence A and 3′ end sequence B of the quantitative detection nucleic acid fragment are the same as or different from the corresponding 5′ end sequence A′ and 3′ end sequence B′ of the nucleic acid target sequence of the test sample;
• the sequence A consists of a 5′ end primer sequence for amplifying the nucleic acid target sequence of the test sample and two bases of the nucleic acid target sequence of the test sample directly downstream of the 5′ end primer sequence;
• and the sequence B consists of a 3′ end primer sequence for amplifying the nucleic acid target sequence of the test sample and two bases of the nucleic acid target sequence of test sample directly upstream of the 3′ end primer sequence;
• the base sequence between the 5′end sequence A and 3′ end sequence B of the quantitative detection nucleic acid fragment and the base sequence between the 5′end sequence A′ and 3′ end sequence B′ of the nucleic acid target sequence of test sample are completely different.