Back to results
US 12,637,712 B2Granted
Fragmentation of DNA
AssigneeNew England Biolabs, Inc.
InventorsLynne Apone, Brittany S. Sexton, Margaret Heider, Louise Js Williams, Eileen T. Dimalanta
Life of this patent
Estimating the term…
Abstract
Provided herein is a polymerase-free enzyme mix (FRAG) for fragmenting double-stranded DNA. In some embodiments the enzyme mix may comprise a double-stranded DNA nickase and at least one of a DNA ligase capable of sealing a nick within a DNA, and a single-strand specific DNA nuclease. Methods for fragmenting double-stranded DNA are also provided.
Claims12
A reaction mix comprising:
- (a) a nickase that nicks double-stranded DNA;
- (b) a single-strand DNA nuclease;
- (c) a DNA ligase;
- and
- (d) fragments of double-stranded genomic DNA, wherein the fragments have a median length in the range of 100 bp to 40 kbp.
2 more elements
The reaction mix according to 1, further comprising:
- one or more of a polynucleotide kinase (PNK), a nicking agent, and a DNA repair enzyme.
Dependencies are inferred from the claim text.