Direct electrical readout of nucleic acid sequences
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsStuart Lindsay, Bintian Zhang, Hanqing Deng
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Abstract
Disclosed are devices, systems and methods for direct measurement of polymerase activity. In one example, a device includes at least a first electrode and a second electrode, the first and second electrode being separated by a gap; and a polymerase with two attachment sites, one for attaching to the first electrode and a second for attaching to the second electrode, wherein the two attachment sites are separated by a distance of at least about 1 nm and the distance does not significantly change with conformational changes of the polymerase.
Claims29
A device for sequencing nucleic acids, comprising:
- at least a first electrode and a second electrode, the first and second electrode are separated by a gap;
- and
- a polymerase comprising two attachment sites, a first attachment site for attachment of the polymerase to the first electrode and a second attachment site for attachment of the polymerase to the second electrode, wherein at least the first attachment site of the polymerase is specifically chemically modified for attachment to the first electrode;
- wherein the two attachment sites are separated by a distance of at least about 1 nm and when the polymerase is attached to the first and second electrodes, the first attachment site and the second attachment site do not undergo movement relative to each other that exceeds 10% of their separation when the polymerase undergoes open-to-closed conformational changes, and wherein the two attachment sites of the polymerase are in an inactive region of the polymerase.
Dependencies are inferred from the claim text.