Method and apparatus for generating droplet array on microfluidic chip

A method for generating a droplet array on a microfluidic chip,

• wherein the microfluidic chip includes an upper chip and a lower chip, wherein a fluid tube of the upper chip is a structure containing a one or more connected fluid channels;
• and the lower chip is provided with a microwell array;
• wherein a lower surface of the upper chip and an upper surface of the lower chip are in contact with each other;
• wherein, when the upper chip and the lower chip are assembled to a initial position, the fluid tube of the upper chip completely covers the microwell array of the lower chip;
• the upper chip or the lower chip is provided with a liquid inlet hole, and the upper chip or the lower chip may also be provided with a liquid outlet hole;
• and the method includes the following steps:
• Step 1. Assembling the upper chip and the lower chip to the initial position, the fluid tube of the upper chip completely covers the microwell array of the lower chip, and the fluid tube of the upper chip is the structure containing the one or more connected fluid channels;
• Step 2. Injecting solution into the microfluidic chip, and the solution partially or completely fills the microwell array of the lower chip;
• Step 3. Moving the upper chip and the lower chip relatively to a liquid splitting position, the fluid tube of the upper chip and the microwell array of the lower chip no longer overlap, and the solution is dispersed into the microwell array to form the droplet array;
• wherein the droplet array is an array containing 1000 or more microdroplets.