Composition and method for improving detection of biomolecules in biofluid samples

A method of detecting a nucleic acid in a biofluid sample, the method comprising:

• (a) adding to the biofluid sample a composition comprising:
• (i) a sufficient amount of dextran sulphate to provide between 50 nM and 5 μM dextran sulphate when the composition is added to the biofluid sample;
• (ii) a buffer agent comprising a sufficient amount of Tris-HCI (pH 7.0) to provide between 8 mM and 800 mM Tris-HCI (pH 7.0) when the composition is added to the biofluid sample;
• (iii) a chelating agent comprising a sufficient amount of ethylenediaminetetraacetic acid (EDTA) to provide between 0.4 mM and 40 mM EDTA when the composition is added to the biofluid sample;
• (iv) a sufficient amount of boric acid to provide between 4 mM and 400 mM boric acid when the composition is added to the biofluid sample;
• (v) a sufficient amount of sodium citrate to provide between 4 mM and 400 mM sodium citrate when the composition is added to the biofluid sample;
• and
• (vi) a sufficient amount of lithium chloride to provide between 1 mM and 100 mM lithium chloride when the composition is added to the biofluid sample;
• and
• (b) detecting the nucleic acid by polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP), recombinase polymerase amplification (RPA), helicase-dependent isothermal DNA amplification (HDA), Southern blotting, quantitative reverse-transcription PCR, capillary sequencing, fragment analysis, Next Generation Sequencing (NGS), or Northern blotting.