Nucleic acid molecules, polypeptides having epoxy group-removing catalytic activity and use thereof
AssigneeShandong Agricultural University
InventorsLingrang Kong, Hongwei Wang, Silong Sun, Wenyang Ge, Bingqian Hou
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Abstract
Disclosed are a nucleic acid molecule, a polypeptide having epoxy group-removing catalytic activity and use thereof. According to the invention, by means of genetic engineering, the nucleic acid molecule encoding a de-epoxidation protein is expressed in a plant, so that an epoxy group of a trichothecene mycotoxin is removed, and the toxin amount in the plant is reduced. The polypeptide of the invention is capable of catalyzing a reaction between vomitoxin and glutathione under mild conditions to remove epoxy groups to produce a glutathionylated derivative.
Claims3
A method for plant transgenesis so as to control a disease, comprising:
- introducing a nucleic acid into a host plant;
- allowing the nucleic acid to be expressed, and thereby obtaining a polypeptide;
- expressing the polypeptide and thereby controlling diseases caused by infection with Fusarium species, wherein the nucleic acid molecule has a sequence selected from the group consisting of the following (a) and (b):
- (a) a sequence as set forth in SEQ ID NO:
- 1;
- and (b) a sequence that is codon-optimized for expression in the host plant, having 90% or more sequence identity to (a), and encoding a polypeptide having an amino acid sequence as set forth in SEQ ID NO:
- 36, wherein the host plant is wheat or maize receiving the introduced nucleic acid.
A plant cell, comprising an exogenous nucleic acid molecule introduced by means of genetic engineering, wherein the exogenous nucleic acid molecule has a sequence selected from the group consisting of the following (a) and (b):
- (a) a sequence as set forth in SEQ ID NO:
- 1;
- and (b) a sequence that is codon-optimized for expression in the host plant, having 90% or more sequence identity to (a), and encoding a polypeptide having an amino acid sequence as set forth in SEQ ID NO:36, wherein the host plant is wheat or maize.
Dependencies are inferred from the claim text.