Recombinant host cells for the production of malonate

A method of isolating dialkyl malonate comprising the steps of:

• culturing a recombinant host cell selected from Pichia kudriavzevii and Yarrowia lipolytica comprising a malonyl-CoA hydrolase, which is a mutant Pseudomonas fulva (strain 12-X) protein of SEQ ID NO:50, wherein the mutant Pseudomonas fulva protein comprises the mutations:
• E95N, V152A, and F304R in a fermentation broth under conditions that result in malonate production;
• separating the host cells from the fermentation broth;
• adding an aliphatic alcohol and acid catalyst to the fermentation broth to form a dialkyl malonate;
• and
• separating the resulting dialkyl malonate from the fermentation broth to provide isolated dialkyl malonate.