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US 12,637,679 B2Granted
Aptamer based system to quantify anti-thrombin III in blood
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsBruce Davis Spiess, Weihong Tan
Life of this patent
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Abstract
Compounds and compositions for determining the level of antithrombin (ATIII) in a sample are described. Methods of forming the compounds and compositions are also described. Methods of using the compounds and compositions to quantify the level of ATIII in a subject are further described. The methods can be used to facilitate determining a dosage or heparin or ATIII to administer to a patient.
Claims18
A kit for determining the level of anti-thrombin (ATIII) in a subject, comprising:
- (a) a first signaling aptamer comprising a first thrombin-specific aptamer comprising the nucleotide sequence of SEQ ID NO:1, a first hybridization sequence, and a fluorescent label;
- and
- (b) a second signaling aptamer comprising a second thrombin-specific aptamer comprising the nucleotide sequence of SEQ ID NO:2, a second hybridization sequence, and a quencher,
- wherein the first and second hybridization sequences are complementary to each other, and wherein fluorescence of the fluorescent label is quenched when the first and second thrombin-specific signaling aptamers are bound to thrombin.
1 more element
A complex comprising:
- a thrombin;
- a first signaling aptamer comprising a first thrombin-specific aptamer comprising the nucleotide sequence of SEQ ID NO:
- 1, a first hybridization sequence, and a fluorescent label;
- and a second signaling aptamer comprising a second thrombin-specific aptamer comprising the nucleotide sequence of SEQ ID NO:
- 2, a second hybridization sequence, and a quencher, wherein the first and second hybridization sequences are complementary to each other and form a duplex, and wherein fluorescence of the fluorescent label is quenched.
2 more elements
Dependencies are inferred from the claim text.