Oncolytic vaccinia virus

A vaccinia virus deficient in functions of vaccinia virus growth factor (VGF), extracellular signal-regulated kinase (ERK)-activating protein, and ribonucleotide reductase (RNR), wherein all or part of regions of each of C11R, O1L, and F4L genes have been deleted or modified, and the functions of these gene products have been inactivated;

• wherein genes encoding extracellular enveloped virus (EEV)-related proteins are replaced by a corresponding genes of another vaccinia virus strain having a high EEV productivity;
• wherein the genes encoding the extracellular enveloped virus (EEV)-related proteins are A33R, A36R, A56R, B5R, F12L, and F13L, and have been replaced by corresponding genes of another vaccinia virus strain having a high EEV productivity, wherein the corresponding genes of another vaccinia virus strain having a high EEV productivity are genes encoding the same amino acid sequence as those of EEV-related proteins selected from the group consisting of A33R, A36R, A56R, B5R, F12L, and F13L being present in vaccinia virus IHD-J strain or IHD-W strain;
• or
• wherein the genes encoding the extracellular enveloped virus (EEV)-related proteins are A33R, A34R, A36R, A56R, B5R, F12L, and F13L, and have been replaced by corresponding genes of another vaccinia virus strain having a high EEV productivity, wherein the corresponding genes of another vaccinia virus strain having a high EEV productivity are genes encoding the same amino acid sequence as those of EEV-related proteins selected from the group consisting of A33R, A34R, A36R, A56R, B5R, F12L, and F13L being present in vaccinia virus IHD-J strain or IHD-W strain.