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US 12,637,658 B2Granted
Method for gene transfer into gamma-delta type T cell
AssigneeMie University
InventorsHiroshi Shiku, Yoshihiro Miyahara, Satoshi Okumura, Takuma Kato, Tae Hayashi, Yoshimasa Tanaka
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Abstract
The present invention provides a method for efficiently producing highly pure γδT cells introduced with foreign genes by culturing in the presence of IL-7 and IL-15 after stimulating the γδT cells with a bisphosphonic acid ester derivative. When a T cell receptor (TCR) or a chimeric antigen receptor (CAR) is introduced as a foreign gene, γδT cells in which TCR and CAR are functionally expressed can be obtained.
Claims8
A method for producing a γδT cell population expressing a transgene, which comprises
- Step 1:
- culturing γδT cells in the presence of tetrakispivaloyloxymethyl 2-(thiazole-2-ylamino) ethylidene-1,1-bisphosphonate or a pharmaceutically acceptable salt thereof;
- Step 2:
- washing the γδT cells cultured in step 1 by centrifugation;
- Step 3:
- culturing the γδT cells washed in step 2 in the presence of IL-7 and IL-15;
- and
- Step 4:
- introducing a gene into the γδT cell cultured in step 3, wherein the gene is NY-ESO-1-specific TCR, HTLV-1 Tax-specific TCR, MAGE-A4-specific TCR, MAGE-A4-specific CAR, CEA-specific CAR, or GD2-specific CAR.
6 more elements
Dependencies are inferred from the claim text.