Methods and materials for culturing, proliferating, and differentiating stem cells
AssigneeMayo Foundation for Medical Education and Research
InventorsAlan D. Marmorstein, Jarel K. Gandhi, Travis J. Knudsen, Matthew S. Hill, Jose S. Pulido
Life of this patent
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Abstract
This document provides RPE cells and RPE monolayers. For example, compositions containing RPE cells or RPE monolayers as well as methods and materials for making RPE cells or RPE monolayers from, for example, stem cells (e.g., iPSCs) are provided.
Claims6
A method for making, from stem cells, a flat retinal pigment epithelium monolayer within a container, wherein said method comprises culturing stem cells in a container having a surface coated with soluble and clottable fibrinogen and comprising a medium capable of differentiating said stem cells into retinal pigment epithelium cells capable of forming said flat retinal pigment epithelium monolayer, wherein said surface was coated with about 3 μg/mL to about 1000 μg/mL of said soluble and clottable fibrinogen, wherein said stem cells are in contact with said soluble and clottable fibrinogen, wherein said stem cells differentiate into said retinal pigment epithelium cells in said container, and wherein said retinal pigment epithelium cells form said flat retinal pigment epithelium monolayer in said container.
Dependencies are inferred from the claim text.