Measuring deformability of a cell
AssigneeHewlett-Packard Development Company, L.P.
InventorsFausto D'Apuzzo, Viktor Shkolnikov, Alexander Govyadinov
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Abstract
An example method for measuring deformability of a cell, consistent with the present disclosure, includes detecting a single cell of a biologic sample in a cell probing chamber of a microfluidic device. The method includes isolating the cell in the cell probing chamber of the microfluidic device by terminating the flow of the biologic sample through the microfluidic device. The method further includes causing deformation of the cell by introducing ultrasonic waves into the cell probing chamber, and measuring deformability of the cell responsive to the introduction of the ultrasonic waves.
Claims10
An apparatus, comprising:
- a fluidic channel actuated by a set of fluidic pumps;
- a cell probing chamber disposed in the fluidic channel, wherein the cell probing chamber is to hold a single cell from a biologic sample for deformation testing;
- and
- an ultrasound source acoustically coupled to the cell probing chamber through a via coupled with the cell probing chamber, the ultrasound source configured to perform deformation testing on the cell by applying, through the via, a pressure field to the cell in the cell probing chamber,
- wherein the via is formed through a silicon top layer of the apparatus, the via to fill with an aqueous acoustic transmission medium and terminating at the cell probing chamber without extending into a base layer of the apparatus.
An apparatus, comprising:
- a lateral fluidic channel and a longitudinal fluidic channel disposed orthogonal to the lateral fluidic channel, wherein each of the lateral fluidic channel and the longitudinal fluidic channel are actuated by a different respective set of fluidic pumps;
- a cell probing chamber disposed at an intersection of the lateral fluidic channel and the longitudinal fluidic channel, wherein the cell probing chamber is to hold a single cell from a biologic sample for deformation testing;
- an ultrasound source acoustically coupled to the cell probing chamber through a via coupled with the cell probing chamber, the ultrasound source configured to perform deformation testing on the cell by applying, through the via, a pressure field to the cell in the cell probing chamber;
- and
- a plurality of channels fluidically coupled to the cell probing chamber to sort cells after deformation testing.
Dependencies are inferred from the claim text.