Method for producing peptide containing non-natural amino acid
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsSakiko Yoshii, Koichiro Shimomura, Sou Ishino
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Abstract
The present invention revealed that translating an mRNA that encodes a peptide containing an unnatural amino acid in a translation system that contains a ribosome containing an engineered L31 protein can increase the amount of the translated peptide. Furthermore, the invention revealed that by using this ribosome, the relative amount of by-products can also be reduced. An engineered L31 protein of the present invention has an amino acid sequence with deletion of 6 or more amino acid residues from the C terminus in the amino acid sequence of the wild-type Escherichia coli L31 protein.
Claims5
A method for producing a peptide, comprising a step of translating an mRNA encoding a peptide comprising one or more types of unnatural amino acids in a translation system that comprises a ribosome comprising an engineered L31 protein, wherein the engineered L31 protein is selected from the group consisting of the proteins of (1) to (3) below:
- (1) a protein comprising an amino acid sequence with deletion of 6 to 50 amino acid residues from the C terminus in the amino acid sequence set forth in SEQ ID NO:
- 1;
- (2) a protein comprising an amino acid sequence in which 1, 2, 3, 4, or 5 amino acid(s) are inserted, substituted, deleted and/or added compared to the amino acid sequence of the protein of (1);
- and
- (3) a protein comprising an amino acid sequence having 95% or more sequence identity to the amino acid sequence of the protein of (1);
- and
- wherein the ribosome comprising the protein of (2) and (3) has greater activity for translation of the peptide comprising the unnatural amino acid(s), as compared to the ribosome comprising the wild-type Escherichia coli L31 that comprises the amino acid sequence of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.