US 12,636,615 B2Granted

Separation apparatus and method

AssigneeThe Penn State Research Foundation

InventorsAndrew Lawrence Zydney, Christopher J. Yehl

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Abstract

Protein separation can be provided based on a High Performance Countercurrent Membrane Purification (HPCMP), which can exploit highly selective diffusive transport across thin walls of a hollow fiber membrane for separation of proteins. Embodiments of an HPCMP system can separate mixtures of multiple proteins (e.g. separate BSA and Mb) or other biological material and provide high yields (e.g. achieving greater than 98% yield of both proteins with purification factors greater than 100-fold, etc.). Embodiments of a HPCMP system can be configured for high resolution separations in the preparation of biopharmaceuticals and natural protein products. Other embodiments can be utilized in other protein separation environments or biological material separation environments.

Claims21

Dependent claims

A method of separating proteins comprising:

passing a feed to a High Performance Countercurrent Membrane Purification (HPCMP) module having at least one membrane within a vessel, the feed comprising a first protein and a second protein, the feed passed through the HPCMP module in countercurrent flow with a buffer fed to the vessel of the HPCMP module;
separating the first protein from the second protein via differences in diffusive flux across the at least one membrane of the HPCMP module;
outputting a product flow and a buffer outlet flow from the vessel of the HPCMP module, the product flow having a purity of the first protein that is greater than the purity of the first protein within the feed, the buffer outlet flow having a purity of the second protein that is greater than the purity of the second protein within the feed passed to the HPCMP module;
wherein the passing of the feed and the outputting of the product flow and the buffer outlet flow occur simultaneously during a continuous flow operation of the HPCMP module.

1 more element

A method of separating biological material comprising:

passing a feed to a High Performance Countercurrent Membrane Purification (HPCMP) module having at least one membrane within a vessel, the feed comprising a first desired biological product and at least one second component, the feed passed through the HPCMP module in countercurrent flow with a buffer fed to the vessel of the HPCMP module;
separating the first desired biological product from the second component via differences in diffusive flux across the at least one membrane of the HPCMP module;
and
outputting a product flow and a buffer outlet flow from the vessel of the HPCMP module, the product flow having a purity of the first desired biological product that is greater than the purity of the first desired biological product within the feed, the buffer outlet flow having a purity of the at least one second component that is greater than the purity of the at least one second component within the feed passed to the HPCMP module;
wherein the passing of the feed and the outputting of the product flow and the buffer outlet flow occur simultaneously during a continuous flow operation of the HPCMP module.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application: 18/262,989Filed 30 Aug 2021 · us provisional application
Priority: 26 Feb 2021
Published: US 2024/0101597 A128 Mar 2024
Granted: 26 May 2026
Term ends: Not estimatedThe source records don’t support an estimate.

Classification11 codes

B01D 61/243
Dialysis ; Membrane extraction: dialysis
B01D 61/16
Ultrafiltration; Microfiltration: feed pretreatment
B01D 61/18
Ultrafiltration; Microfiltration: apparatus therefor
B01D 61/58
Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary…