Continuous purification device

A method of purification of a substance of interest, comprising:

• directing a first liquid comprising the substance of interest into a mixing module of an apparatus,
• directing a second liquid comprising a plurality of particles into the mixing module of the apparatus, and
• flowing the first liquid and the second liquid through the apparatus,
• wherein the apparatus comprises:
• i) the mixing module comprising:
• a first network comprising a first plurality of gyroids configured in layers to provide a first plurality of microchannels, wherein the first plurality of microchannels are fluidically interconnected;
• ii) a first port configured to receive the first liquid, wherein the first port comprises a first plurality of sub-ports, each sub-port is in fluid communication with an inlet of a microchannel of a first layer of the first plurality of gyroids;
• iii) a second port configured to receive the second liquid, wherein the second port comprises a second plurality of sub-ports, each sub-port is in fluid communication with an inlet of a microchannel of the first layer of the first plurality of gyroids;
• iv) a wash module downstream of the mixing module comprising:
• a second network comprising a second plurality of gyroids in fluid communication with the mixing module and configured to provide a second plurality of microchannels, wherein the second plurality of microchannels are fluidically interconnected;
• v) a third port configured to receive a third liquid, wherein the third port is upstream of the wash module;
• vi) a first separation module downstream of the wash module comprising:
• at least one channel in fluid communication with the wash module;
• a fourth port configured to receive a fourth liquid comprising an elution solution;
• a first transport component configured to interact with the plurality of particles, thereby directing the plurality of particles to the fourth liquid in the at least one channel;
• and
• a first outlet and a second outlet each in fluid communication with the at least one channel of the first separation module, wherein the first outlet is configured to receive the fourth liquid and the plurality of particles exiting the wash module and the second outlet is configured to receive remaining liquid;
• vii) an elution module downstream of the first separation module comprising:
• a third network comprising a third plurality of gyroids configured to provide a third plurality of microchannels, wherein the third plurality of microchannels are fluidically interconnected and in fluid communication with the first outlet;
• and
• viii) a second separation module downstream of the elution module comprising:
• at least one channel in fluid communication with the elution module;
• a fifth port configured to receive a fifth liquid comprising a re-equilibration solution;
• a third outlet and a fourth outlet each in fluid communication with the at least one channel of the second separation module, wherein the fourth outlet is configured to receive the re-equilibration solution and the plurality of particles exiting the elution module and the third outlet is configured to receive remaining liquid;
• and
• a second transport component configured to interact with the plurality of particles, thereby directing the plurality of particles to the re-equilibration solution.