Method of implantation of cell aggregates and tissue fragments
AssigneeNeuronano AB
InventorJens Schouenborg
Life of this patent
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Abstract
In a method for implantation of a physically stabilized aggregate of living cells or tissue fragment is injected into a channel provided in soft tissue filled with an aqueous gel. Also discloses are methods of stabilizing such aggregates and fragments and of forming such channel in soft tissue as well as means for carrying out the methods.
Claims12
A system for performing a method for implantation of an implant selected from single living cells, aggregates of living cells, and tissue fragments, such as sheets of tissue with living cells into soft tissue, the system comprising:
- an apparatus having an oblong rigid pin having a front end and a rear end and a layer comprising of dry gel forming agent disposed on a pin section extending from the front end in a distal direction and enclosing said section, wherein said layer or agent contains less than 20% by weight of water, wherein the pin is sufficiently rigid to allow the pin to be inserted into soft tissue in absence of the layer comprising of dry gel forming agent;
- and
- a syringe or pipette, wherein the method comprises,
- i) inserting the pin into soft tissue with the front end thereof foremost;
- ii) allowing a channel filled with aqueous gel to be formed around the pin by contact of gel forming agent with aqueous body fluid;
- iii) withdrawing the pin from the gel,
- iv) loading the implant in the syringe or the pipette;
- v) inserting the syringe or the pipette into the gel;
- vi) expelling the implant from the syringe of pipette into the gel;
- and
- vii) withdrawing the syringe or pipette from the gel, and
- wherein the implant comprises a support selected from any one of:
- sheet of biocompatible material, biocompatible fiber and combinations thereof, and a time difference between the provision of the channel and implantation is at least one minute.
A method for implantation of an implant selected from single living cells, aggregates of living cells and tissue fragments into soft tissue comprising the application of a system, the system comprising:
- an apparatus having an oblong rigid pin having a front end and a rear end and a layer comprising of dry gel forming agent disposed on a pin section extending from the front end in a distal direction and enclosing said section, wherein said layer or agent contains less than 20% by weight of water, wherein the pin is sufficiently rigid to allow the pin to be inserted into nervous tissue in absence of its layer comprising of dry gel forming agent;
- and
- a syringe or pipette, wherein the method comprises,
- i) inserting the pin into soft tissue with its front end foremost;
- ii) allowing a channel filled with aqueous gel to be formed around the pin by contact of gel forming agent with aqueous body fluid;
- iii) withdrawing the pin from the gel,
- iv) loading the implant in the syringe or the pipette;
- v) inserting the syringe or the pipette into the gel;
- vi) expelling the implant from the syringe of pipette into the gel;
- and
- vii) withdrawing the syringe or pipette from the gel.
Dependencies are inferred from the claim text.