System and method for delivery of gas to a tissue

A system for delivery of gas to a tissue, the system comprising:

• a container containing the gas, wherein the gas is gaseous nitric oxide (gNO);
• an applicator, wherein the applicator is configured to deliver the gas to the tissue;
• a three-port valve having a first port for receiving the gas from the container, a second port for receiving a purging gas or connecting to a vacuum source, and a third port in fluid communication with the applicator, the three-port valve being switchable between a first state at which the first port fluidly connects to the third port, and a second state at which the second port fluidly connects to the third port;
• a flow control system for controlling flow of gas exiting the three-port valve;
• a computerized controller for automatically controlling the flow control system, according to at least one of:
• a predetermined gas flow rate, a predetermined total amount of the gas flowing through the flow control system, and a predetermined total amount of time in which the gas flows through the flow control system;
• and
• an adjustable pressure regulator, in fluid communication with the third port of the three-port valve, and being configured for maintaining a pressure which is below a predetermined threshold when the three-port valve assumes the first state, and a pressure which is above the predetermined threshold when the three-port valve assumes the second state;
• wherein a concentration of the gNO in the container is from about 1,000 ppm to about 1,000,000 ppm.