Automated system and methods for tissue demineralization

A system for automatically demineralizing or decellularizing a tissue portion, comprising:

• a control and reporting system in communication with a mixing assembly having a stationary cannister forming an interior mixing chamber configured to receive the tissue portion, a reagent inlet in fluid communication between a plurality of reagent liquid containers and the interior mixing chamber, a reagent outlet in fluid communication between the interior mixing chamber and a waste container, and an agitator rotatively mounted within the interior mixing chamber, wherein the control and reporting system includes a processor communicatively coupled with a data storage storing a processing module, the processor executing the processing module for:
• inputting a measured quantity of a first reagent into the interior mixing chamber via the reagent inlet;
• rotating the agitator within the interior mixing chamber in a first direction relative to the stationary cannister;
• rotating the agitator within the interior mixing chamber in a second direction relative to the stationary cannister;
• repeating the rotating the agitator in the first and the second directions for an agitation time;
• draining the measured quantity of the first reagent from the interior mixing chamber via the reagent outlet;
• repeating the inputting, the rotating the agitator in the first direction, the rotating the agitator in the second direction, the repeating the rotating the agitator in the first and the second directions, and the draining for a measured quantity of a second reagent and a measured quantity of a third reagent;
• during the draining the measured quantity of the third reagent from the mixing chamber, measuring a pH level of the measured quantity of the third reagent;
• and
• when the pH level is less than 6.0, repeating the inputting, the rotating the agitator in the first direction, the rotating the agitator in the second direction, the repeating the rotating the agitator in the first and the second directions, and the draining for the measured quantity of the first reagent, the measured quantity of the second reagent, and the measured quantity of the third reagent.