Biological prosthesis and methods of production and use
AssigneeRosivo LLC
InventorEran Rosines
Life of this patent
Estimating the term…
Abstract
Products, processes, compositions, kits, and methods are provided for cartilage-derived implants. The implants can exhibit resistance to enzyme (e.g., collagenase, protease, etc.) digestion compared to the source tissue from which they were derived while still having one or more mechanical properties comparable to the source tissue from which they were derived. The implants can also have a plurality of molecular bridges between molecules of the cartilaginous material. The molecular bridges can connect one or more collagen fibrils and/or/with one or more glycosaminoglycans. The implants can also be treated with cationic detergent, packaged and sterilized with or without additional components, and surgically implanted into subjects.
Claims27
A cartilage-derived tissue composition, comprising:
- a cartilage-derived body comprising intact extra-cellular matrix and one or more molecular bridges between molecules of the extra-cellular matrix, the one or more molecular bridges selected from the group consisting of:
- a molecular bridge formed between amine groups of one or more collagen fibrils;
- a molecular bridge formed between hydroxyl groups of one or more glycosaminoglycan molecules;
- a molecular bridge formed between a hydroxyl group of a glycosaminoglycan molecule and an amine group of a collagen fibril;
- a sulfone-containing bridge;
- and
- a bridge formed from treating the cartilage with a hydrocarbon diol reactive molecule having between 3 and 12 carbon atoms.
A cartilage-derived tissue composition, comprising:
- an enzymatically resistant cartilage-derived body comprising intact extra-cellular matrix obtained by treating source cartilage with at least one multifunctional reactive molecule configured to form one or more molecular bridges between molecules of the source cartilage, the one or more molecular bridges selected from the group consisting of:
- a molecular bridge formed between amine groups of one or more collagen fibrils;
- a molecular bridge formed between hydroxyl groups of one or more glycosaminoglycan molecules;
- a molecular bridge formed between a hydroxyl group of a glycosaminoglycan molecule and an amine group of a collagen fibril;
- a sulfone-containing bridge;
- and
- a bridge formed from treating the cartilage with a hydrocarbon diol reactive molecule having between 3 and 12 carbon atoms.
A cartilage-derived tissue composition, comprising:
- a cartilage-derived body comprising intact extra-cellular matrix obtained by treating source cartilage with at least one multifunctional reactive molecule is selected from the group consisting of a molecule comprising more than one and less than 5 reactive epoxide groups;
- divinyl sulfone;
- 1,4-butanediol diglycidyl ether (BDDE);
- 1,2,7,8-diepoxyoctane;
- and combinations thereof.
Dependencies are inferred from the claim text.