Recombinant non-structural protein 1, recombinant influenza virus and immunological composition including the same, and method of treating or preventing disease or condition caused by or associated with influenza virus

A recombinant NS protein 1, characterized that an amino acid sequence of the recombinant NS protein 1 corresponding to the 1st to the 4th amino acid residues of TIMs of wild-type NS 1 is encoded by a nucleic acid sequence selected from the group consisting of any one listed as SEQ ID NOs:

• 8 and 9, and the 1st to the 4th amino acid residues of the TIMs of the wild-type NS 1 comprises the Ind to the 4th amino acid residues of an amino acid sequence listed as SEQ ID NO:
• 1.

A recombinant influenza virus comprising hemagglutinin (HA), neuraminidase (NA), nucleoprotein (NP), matrix (M) protein, polymerase basic protein 1 (PB1), PB2, polymerase acidic protein 1 (PA) and a recombinant NS protein 1, which is characterized that the recombinant NS protein 1 comprises an amino acid sequence listed as any one of SEQ ID NOs:

• 55 and 60, at least one amino acid residue of the PB2 and the M protein has at least one site mutation, and the at least one amino acid residue of the NS protein 1 has site mutation except for the amino acid sequence.