In vitro heparin and heparan sulfate compositions and methods of making and using
AssigneeTEGA Therapeutics, Inc.
InventorsCharles Glass, Bryan Thacker, Jeffrey Esko
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Abstract
Disclosed herein are cellular derived heparin and heparan sulfate compositions, methods of making and using, and cell lines for making and using.
Claims8
A composition comprising a heparan sulfate, wherein the composition is at least 99.5% free of chondroitin sulfate, wherein the composition is derived from a cell line comprising a genetic deficiency in Heparan sulfate 2-O-sulfotransferase (HS2ST) and chondroitin sulfate synthase 1 (ChSy1) and transgenic for Sulfatase 2 (Sulf2), and wherein the composition has reduced binding to Platelet Factor 4 (PF4), and/or Vascular Endothelial Growth Factor (VEGF) compared with heparin or heparan sulfate isolated from a wildtype Chinese Hamster Ovary (CHO) cell.
Dependencies are inferred from the claim text.