Back to results
US 12,635,709 B2Granted
Method for separation of proteins naturally occurring in mammalian milk
AssigneeLihme Protein Solutions ApS
InventorsAllan Otto Fog Lihme, Marie Bendix Hansen
Life of this patent
Estimating the term…
Abstract
The present invention relates to method for purifying proteins naturally occurring in mammalian milk from aqueous protein solution using water-soluble silicate. The silicates form an insoluble precipitate of a silicate-protein complex from which the proteins can be isolated.
Claims21
A method for isolating one or more proteins naturally occurring in mammalian milk from an aqueous protein solution comprising said one or more proteins and impurities and being selected from the group consisting of milk and whey and concentrates and derivatives thereof, the method comprising:
- A. providing an aqueous solution containing the one or more proteins and the impurities, wherein said aqueous solution is selected from the group consisting of milk and whey and concentrates and derivatives thereof,
- B. adding a water-soluble silicate to the solution of step (A) such that the total concentration of silicon in the form of free or complexed silicates in the solution is in the range of 1-750 mM,
- C. if necessary, adjusting the pH of the resulting solution to a pH in the range of pH 1 to pH 11,
- D. allowing the silicate to form an insoluble precipitate of a silicate-protein complex,
- E. separating the silicate-protein complex from the solution as a wet precipitate;
- and
- F. dissociating the one or more proteins from the silicate-protein complex,
- thereby obtaining the isolated protein product.
5 more elements
Dependencies are inferred from the claim text.