Aqueous hop extracts and their use as antimicrobial agents
AssigneeNature Recombined Sciences Inc.
InventorsLeonard Sarna, Kian-Guan Lim, Mingyang Sun, Silvia Cardona, John Sorensen, Anna Motnenko
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Abstract
Described herein is the preparation of an aqueous hop extract and a boiling water hop extract prepared from dried hop cones that were ground prior to extraction. As discussed below, extracts were prepared from a number of different hop varieties and were tested against a number of clinically relevant bacterial strains. As discussed herein, surprisingly, the boiling water extract showed greater antibacterial activity than the aqueous extract.
Claims6
A method of treating a microbially contaminated hospital or care home surface comprising:
- applying an effective amount of a disinfectant composition to the microbially contaminated hospital or care home surface selected from the group consisting of a floor, a countertop or a curtain, the active ingredient of the disinfectant composition prepared by a method comprising:
- grinding a quantity of hop cones from hop plants,
- suspending the ground hop cones in a solution consisting of water, thereby providing forming an aqueous suspension;
- boiling the aqueous suspension for at least about 15, 20 or 30 minutes, and
- recovering the supernatant, thereby providing a boiling water hop extract;
- said disinfectant composition arresting microbial growth on the microbially contaminated hospital or care home surface, wherein the microbial growth is from contamination by a bacterium selected from the group consisting of:
- Staphylococcus aureus, Enterococcus faecium, Listeria monocytogenes, Methicillin-Resistant Staphylococcus aureus (MRSA) and Streptococcus pneumonia and wherein the effective amount yields at least a log10 biofilm reduction in colony forming units of the bacterium relative to a saline control.
A method of treating a Gram-positive bacterial skin infection in a patient in need of such treatment comprising administering an effective amount of a boiling water hop extract prepared by:
- grinding a quantity of hop cones from hop plants,
- suspending the ground hop cones in a solution consisting of water, thereby providing forming an aqueous suspension;
- boiling the aqueous suspension for at least about 15, 20 or 30 minutes,
- recovering the supernatant, thereby providing the boiling water hop extract,
- to a person who is suspected of having or who has been diagnosed with a Gram-positive bacterial skin infection, wherein the Gram-positive bacteria is selected from the group consisting of Cutibacterium acnes, S. aureus, MRSA Bacillus subtilis, Vancomycin-Resistant Enterococcus faecium (VRE) and L. monocytogenes,
- and wherein the effective amount yields at least a log10 biofilm reduction in colony forming units of the bacterium relative to a saline control.
A method of eradicating a biofilm produced by a Gram-positive bacteria comprising
- applying an effective amount of a disinfectant composition to a microbially contaminated surface comprising a Gram-positive bacteria biofilm, the active ingredient of the disinfectant composition prepared by a method comprising:
- grinding a quantity of hop cones from hop plants,
- suspending the ground hop cones in a solution consisting of water, thereby providing forming an aqueous suspension;
- boiling the aqueous suspension for at least about 15, 20 or 30 minutes, and
- recovering the supernatant, thereby providing a boiling water hop extract;
- said disinfectant composition eradicating the biofilm on the microbially contaminated surface, wherein the biofilm is produced by a Gram-positive bacterium selected from the group consisting of:
- S. aureus, E. faecium, L. monocytogenes, MRSA, VRE and S. pneumonia and wherein the effective amount yields at least a log10 biofilm reduction in colony forming units of the bacterium relative to a saline control.
Dependencies are inferred from the claim text.