Methods of detecting markers for inflammatory conditions and food sensitivity in companion animals
AssigneeVetica Labs, Inc.
InventorsJuan Estruch, Genevieve Hansen
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Abstract
The present invention provides methods and systems to accurately detect and measure levels of endogenous antibodies, for examples endogenous IgA, to particular antigens in a biological sample from a companion animal, which is useful to diagnose inflammatory conditions, including bowel disease (IBD), gastrointestinal infections, and food sensitivities in companion animals, e.g., dogs or cats, and to distinguish among such gastrointestinal disorders. Such methods and systems identify whether a sample from the patient is associated with an inflammatory condition, infection, and/or food sensitivity condition, by using non-invasive means, thus conveniently providing information useful for guiding treatment decisions.
Claims11
A method for detecting the presence and/or level of one or more endogenous antibodies associated with food sensitivity in a serum sample obtained from a companion animal patient,
- wherein the one or more endogenous antibodies are IgA antibodies that bind to one or more sequences selected from the group consisting of SEQ ID NOS 37, 38, 39, and 40,
- and
- wherein the companion animal is a dog;
- the method comprising the steps of
- a. contacting one or more antigens with said sample, wherein the one or more antigens comprise antigenic regions of the one or more proteins associated with food sensitivity, bind to the endogenous antibody of interest, and wherein the one or more antigens are bound to a substrate or detectable label;
- and
- b. detecting the binding of said one or more endogenous antibodies associated with food sensitivity to the one or more antigens;
- wherein the one or more antigens bound to a substrate or detectable label consist of one or more bait sequences selected from the group consisting of SEQ ID NOS 37, 38, 39, 40, 58, and fusion peptides consisting of two or more sequences of SEQ ID NOS 37, 38, 39, and 40, wherein said bait sequences are optionally attached to an N-terminal hexa-histadine tag of SEQ ID NO:
- 36.
Dependencies are inferred from the claim text.