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US 12,631,648 B2Granted
Compounds for the detection of homocysteine and its method of preparation thereof
AssigneeCouncil of Scientific and Industrial Research
InventorsPabitra Baran Chatterjee, Snehasish Debnath, Ratish Rajgopalan Nair
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Abstract
The present invention discloses compounds of Formula-A and its copper complex of general Formula-B. The compound of general Formula-B is used in the selective detection of homocysteine in aqueous medium at physiological pH without any interference from other challenging amino acids and thiols. Using compounds of general Formula-B, specific detection of homocysteine under UV light in aqueous solution is also possible by the naked eyes.
Claims7
A compound of Formula-B
- wherein R1=—CH3 or —H and R2=—CH3 or —C2H5.
A process for preparation of the compound of Formula-B
- wherein R1=—CH3 or —H and R2=—CH3 or —C2H5,
- comprising the steps of:
- (i) refluxing a mixture of 7-Hydroxy-4-methyl-2-oxo-2H-chromene-8-carbaldehyde or 7-Hydroxy-2-oxo-2H-chromene-8-carbaldehyde and N,N-dimethylethylenediamine or N,N-diethylethylenediamine in a stoichiometry ratio 1:1 in a solvent for a period in the range of 3-4 h followed by evaporating the solvent to obtain a residue;
- (ii) triturating the residue as obtained in step (i) with diethyl ether to obtain a yellow solid;
- (iii) recrystallizing the solid as obtained in step (ii) to obtain crystalline Compound of Formula-A;
- (iv) refluxing a mixture of copper salt and compound of Formula-A in 1:1 stoichiometry in a solvent for a period in the range of 3-4 h followed by evaporating the solvent to obtain green coloured solid;
- (v) filtering the green coloured solid as obtained in step (i) followed by drying to obtain a solid;
- and
- (vi) recrystallizing the solid as obtained in step (ii) to obtain compound of Formula-B in pure form.
6 more elements
Dependencies are inferred from the claim text.