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US 12,631,635 B2Granted
Species-specific antigen sequences for tick-borne relapsing fever (TBRF) and methods of use
AssigneeID-Fish Technology, Inc.
InventorsJyotsna S. Shah, Song Liu
Life of this patent
Estimating the term…
Abstract
The disclosure, in some aspects, provides antigen-specific amino acid sequences for tick-borne relapsing fever Borrelia species.
Claims9
A composition comprising one or more labeled and/or bound amino acid sequences selected from the group consisting of:
- SEQ ID NO:
- 1;
- SEQ ID NO:
- 2;
- SEQ ID NO:
- 3;
- SEQ ID NO:
- 4;
- SEQ ID NO:
- 5 and variants thereof having at least 90% homology;
- SEQ ID NO:
- 6 and variants thereof having at least 90% homology;
- SEQ ID NO:
- 7;
- SEQ ID NO:
- 8;
- SEQ ID NO:
- 9;
- SEQ ID NO:
- 10;
- and
- SEQ ID NO:
- 11 and variants thereof having at least 96% homology;
- wherein, when labeled, the one or more amino acid sequences are labeled with biotin/streptavidin, enzyme conjugates, fluorescent moieties, or biological fluorophores,
- wherein, when bound, the one or more amino acid sequences are bound to a substance selected from the group consisting of nitrocellulose, nylon, polyvinylidene difluoride (PVDF), magnetic beads, and agarose.
22 more elements
A method for detecting antibodies resulting from infection by one or more members of Tick-Borne Relapsing Fever (TBRF) Borrelia genus if present in a biological sample from a subject suspected of having TBRF, the method comprising:
- providing the biological sample obtained from the subject suspected of having TBRF;
- contacting the biological sample with a composition comprising one or more labeled and/or bound amino acid sequences selected from the group consisting of:
- SEQ ID NO:
- 1;
- SEQ ID NO:
- 2;
- SEQ ID NO:
- 3:
- SEQ ID NO:
- 4;
- SEQ ID NO:
- 5 and variants thereof having at least 90% homology;
- SEQ ID NO:
- 6 and variants thereof having at least 90% homology;
- SEQ ID NO:
- 7;
- SEQ ID NO:
- 8;
- SEQ ID NO:
- 9;
- SEQ ID NO:
- 10;
- and
- SEQ ID NO:
- 11 and variants thereof having at least 96% homology;
- wherein, when labeled, the one or more amino acid sequences are labeled with biotin/streptavidin, enzyme conjugates, fluorescent moieties, or biological fluorophores,
- wherein, when bound, the one or more amino acid sequences are bound to a substance selected from the group consisting of nitrocellulose, nylon, polyvinylidene difluoride (PVDF), magnetic beads, and agarose;
- and
- detecting a positive immunobinding reaction which indicates the presence of one or more TBRF specific antibodies in the biological sample.
26 more elements
Dependencies are inferred from the claim text.