Assay test device and method

A rapid diagnostic device for detecting multiple biomarkers in a bodily fluid sample, comprising:

• a housing;
• at least one test strip disposed within the housing, each test strip comprising:
• a sample receiving zone at a first end configured to receive the bodily fluid sample,
• at least one conjugate pad containing dried particle conjugates specific to at least two distinct biomarkers,
• at least two test zones each containing immobilized capture reagents for detecting one of the distinct biomarkers,
• a control zone for indicating proper test function;
• and
• a barrier extending lengthwise along the at least one test strip, dividing the conjugate pad and the at least two test zones into two separate flow paths, wherein the conjugate pad includes labelled conjugates to two different analytes in each of the two separate flow paths;
• wherein the bodily fluid sample flows along each test strip from the sample receiving zone through the at least one conjugate pad to the test zones and control zone;
• wherein the at least one test strip is configured to detect at least two different biomarkers;
• and
• wherein a positive result in all test zones indicates an overall positive test result for a specific medical condition.