Chromatographic reader devices for biodetection

A reader device comprising:

• a camera board configured to capture image data associated with an output signal area of a biological chromatographic test strip;
• processing circuitry in communication with the camera board, the processing circuitry being configured to:
• perform image analysis of the image data in an aggregated way to discern color changes in each color channel and sum differences to obtain a full-color delta;
• determine, using an intensity of a background fit section represented in the image data captured by the camera board, based on an intensity offset between one or more attributes of the hook line and the intensity of the background fit section, and (iii) an intensity of a test line displayed in the output signal area of the biological chromatographic test strip;
• determine, based on the intensity of the hook line, that a test sample submitted via the biological chromatographic test strip is of a sufficient concentration to perform analysis with respect to a target analyte in the test sample;
• based on the determination that the test sample submitted via the biological chromatographic test strip is of the sufficient concentration to perform the analysis with respect to the target analyte in the test sample, execute a trained model to draw one or more inferences from one or more signals detected from the output signal area to determine a relative intensity between the test line and at least one of the hook line or control line using the intensity of the test line and at least one of the intensity of the hook line or the intensity of the control line;
• and
• determine, based on the relative intensity and based on the full-color delta, a concentration of the target analyte present in the test sample submitted via the biological chromatographic test strip;
• and
• an interface in communication with the processing circuitry, the interface being configured to output data indicative of the concentration of the target analyte determined by the processing circuitry.

An integrated circuit (IC) configured to:

• obtain image data associated with an output signal area of a biological chromatographic test strip;
• perform image analysis of the image data in an aggregated way to discern color changes in each color channel and sum differences to obtain a full-color delta;
• determine, using an intensity of a background fit section represented in the obtained image data, based on an intensity offset between one or more attributes of a hook line and the intensity of the background fit section, and an intensity of a test line displayed in the output signal area of the biological chromatographic test strip;
• determine, based on the intensity of the hook line, that a test sample submitted via the biological chromatographic test strip is of a sufficient concentration to perform analysis with respect to a target analyte in the test sample;
• based on the determination that the test sample submitted via the biological chromatographic test strip is of the sufficient concentration to perform the analysis with respect to the target analyte in the test sample, execute a trained model to draw one or more inferences from one or more signals detected from the output signal area to determine a relative intensity between the test line and at least one of the hook line or control line using the intensity of the test line and at least one of the intensity of the hook line or the intensity of the control line;
• and
• determine, based on the relative intensity and based on the full-color delta, a concentration of the target analyte in the test sample submitted via the biological chromatographic test strip.