Methods of generating organoids for high throughput screening of drugs

A method of determining a toxicity of a pharmaceutically active agent to be administered to a human subject for a pre-selected exposure time for treating a disease or condition, the method comprising:

• incubating a plurality of human organoids in culture with a respective plurality of different concentrations of said agent, wherein said plurality of human organoids comprise less than 20% variability in size, number of cells or both;
• measuring function of said organoids at a time point, thereby providing a plurality of organoid-function values, one for each of said concentrations, wherein a time-period from a beginning of said incubation to said time point corresponds to said pre-selected exposure time of said subject to the agent;
• fitting said plurality of organoid-function values to an S-shaped curve;
• and
• extracting from said S-shaped curve a predicted toxicity value of the agent for said pre-selected exposure time.