Back to results
US 12,631,605 B2Granted
Acoustic mist ionisation and newborn dried blood spot screening
AssigneeMicromass UK Limited
InventorsDonald Mason, Heather Brown
Life of this patent
Estimating the term…
Abstract
A method of newborn screening (“NBS”) is disclosed comprising directing ultrasonic energy or ultrasonic waves into a metabolite or analyte sample derived from a newborn, neonate or infant so as to cause a mist of charged sample droplets or sample ions to be ejected. The charged sample droplets or sample ions are then mass analysed and a determination is made as to whether or not one or more first metabolites or analytes indicative of a disorder or inborn error are present in the sample.
Claims16
A method of newborn screening (“NBS”) comprising:
- preparing a metabolite or analyte sample for analysis by:
- punching, clipping or removing a portion of a dried blood spot (“DBS”) relating to a newborn, neonate or infant and transferring the portion into a sample well of a first sample plate;
- adding an extraction sample or solvent to the DBS portion in the sample well of the first sample plate;
- shaking or agitating the dried blood spot portion and extraction sample or solvent in the sample well of the first sample plate;
- transferring an extract from the sample well of the first sample plate into a sample well of a second sample plate;
- and
- loading or positioning the second sample plate between an acoustic transducer assembly and a collection nozzle or electrode;
- directing ultrasonic energy or ultrasonic waves into the extract in the sample well of the second sample plate so as to cause a mist of charged sample droplets or sample ions to be ejected;
- mass analysing the charged sample droplets or sample ions;
- and
- determining whether or not one or more first metabolites or analytes indicative of a disorder or inborn error are present in the sample.
8 more elements
Dependencies are inferred from the claim text.