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US 12,631,603 B2Granted
Systems and methods for two-dimensional liquid chromatography using size exclusion chromatography as a first dimension
AssigneeWaters Technologies Corporation
InventorsStephan M. Koza, Pamela Iraneta, Hua Yang
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Abstract
Described herein are systems and methods used for carrying out a two-dimensional liquid chromatography process using size exclusion chromatography as a first dimension.
Claims22
A system for performing a two-dimensional liquid chromatography process, the system comprising:
- a first analytical subsystem comprising:
- a size exclusion chromatography column as a first analytical device;
- and
- a first pump fluidly coupled with the size exclusion chromatography column and configured to deliver a sample to the size exclusion chromatography column;
- a second analytical subsystem comprising:
- a second analytical device different from the first analytical device;
- and
- a second pump fluidly coupled with the second analytical device and coupleable with a source of a diluent, wherein the second pump is configured to deliver the diluent from the source of the diluent to an eluent output from the size exclusion chromatography column and then introduce a merged flow of the diluent and the eluent to the second analytical device;
- and
- a mechanism configured to regulate a flow of the eluent outputted from the size exclusion chromatography column to the second analytical device without the use of a trap, the mechanism configured to stop and restart the flow of the eluent from the size exclusion chromatography column to the second analytical device for desired time periods.
7 more elements
A method of performing a two-dimensional liquid chromatography process, the method comprising the steps of:
- introducing a sample to a first analytical device comprising a size exclusion chromatography column for performing a first separation of the sample;
- directing a flow of an eluent output from the size exclusion chromatography column to a second analytical device for performing a second separation of the sample;
- regulating the flow of the eluent output from the size exclusion chromatography column to the second analytical device without the use of a trap, wherein regulating the flow of the eluent comprises stopping the flow of the eluent from the size exclusion chromatography column to the second analytical device and restarting the flow of eluent from the size exclusion chromatography column to the second analytical device after a desired time period;
- introducing a diluent to the flow of the eluent output from the size exclusion chromatography column prior to delivering the eluent and the diluent to the second analytical device;
- and
- starting the second analytical device.
3 more elements
Dependencies are inferred from the claim text.