Cells, tissues, organs, and animals having one or more modified genes for enhanced xenograft survival and tolerance
AssigneeeGenesis, Inc.
InventorsJacob Layer, Wenning Qin, Yinan Kan, Juliet Crabtree, Michele Youd, David Heja, David Angeles Albores, Ranjith Anand, Paola Perrat, Russell Ernst, Violette Paragas
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Abstract
The present disclosure relates to cells, tissues, organs, and/or animals having one or more modified genes for enhanced xenograft survival and/or tolerance. In addition, the present disclosure relates to methods of making and using the cells, tissues, organs, and/or animals having one or more of the modified genes.
Claims39
A genetically modified porcine cell comprising a nucleic acid molecule, wherein the nucleic acid molecule comprises:
- (a) a first polycistronic cassette comprising:
- (i) an endothelial protein C receptor (EPCR) transgene and (ii) a thrombomodulin (THBD) transgene;
- (b) a second polycistronic cassette comprising:
- (i) a tumor necrosis factor a-induced protein 3 (A20) transgene and (ii) a heme oxygenase-1 (HO1) transgene;
- and
- (c) a third polycistronic cassette comprising:
- (i) a cluster of differentiation 46 (CD46) transgene, (ii) a CD55 transgene, and (iii) a CD47 transgene,
- wherein the nucleic acid molecule is integrated at a single genomic locus.
A genetically modified porcine cell comprising a nucleic acid molecule, wherein the nucleic acid molecule comprises:
- (a) a first polycistronic cassette comprising:
- (i) a tissue factor pathway inhibitor (TFPI) transgene, (ii) an endothelial protein C receptor (EPCR) transgene, and (iii) a thrombomodulin (THBD) transgene;
- (b) a second polycistronic cassette comprising:
- (i) (A) a beta 2 microglobulin (B2M) transgene and a human leukocyte antigen E (HLA-E) transgene, or (B) a transgene encoding a B2M-HLA-E fusion;
- and (ii) a cluster of differentiation 47 (CD47) transgene;
- and
- (c) a third polycistronic cassette comprising:
- (i) a CD46 transgene and (ii) a CD55 transgene,
- wherein the nucleic acid molecule is integrated at a single genomic locus.
Dependencies are inferred from the claim text.