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US 12,630,829 B2Granted
Gene encoding cytochrome P450 and use thereof
AssigneeKumiai Chemical Industry Co., Ltd.
InventorsShunsuke Yamamoto, Kiyoshi Kawai
Life of this patent
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Abstract
Metabolic activity is exhibited against a larger number of compounds having different acting properties. A cytochrome P450 gene encodes a protein having an amino acid sequence of any of SEQ ID NOS: 2, 4, 6, and 8.
Claims2
A transformation method comprising the steps of:
- introducing, into a host cell, an expression vector comprising a cytochrome P450 gene encoding a protein comprising the amino acid sequence of SEQ ID NO:2 and an additional gene;
- and selecting, as a transformant, a cell that grows in the presence of at least one inhibitor selected from the group consisting of an acetolactate synthase inhibitor classified as code 2 of Herbicide Resistance Action Committee (HRAC) classifications, a microtubule assembly inhibitor classified as code 3 of HRAC classifications, a photosystem II inhibitor classified as code 6 of HRAC classification, a carotenoid biosynthesis inhibitor in phytoene desaturase (PDS) classified as code 12 of HRAC classifications, a protoporphyrinogen oxidase inhibitor classified as code 14 of HRAC classifications, a dihydropteroate synthase inhibitor classified as code 18 of HRAC classifications, and a 4-hydroxyphenylpyruvate dioxygenase inhibitor classified as code 27 of HRAC classifications.
Dependencies are inferred from the claim text.