Fluorescent proteins and split fluorescent proteins from corynactis californica and methods of use
AssigneeTriad National Security, LLC
InventorsGeoffrey S. Waldo, Hau Thi Bich Nguyen
Life of this patent
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Abstract
Fluorescent proteins and split-fluorescent proteins (SFPs) including split-green fluorescent proteins from Corynactis californica are provided. Nucleic acid molecules encoding the fluorescent proteins and SFPs, vectors, and kits are also provided. Methods of using the fluorescent proteins and SFPs, such as detecting a protein of interest or protein-protein interactions are also provided.
Claims13
An isolated polypeptide comprising a Split Fluorescent Protein (SFP) detector comprising:
- (i) an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 1, wherein amino acid residue 11 is asparagine, amino acid residue 14 is threonine, amino acid residue 43 is serine, amino acid residue 100 is glutamic acid, amino acid residue 104 is threonine, and amino acid residue 152 is proline, and wherein the SFP detector complements with an SFP tag to form a functional SFP;
- or
- (ii) an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 3, wherein amino acid residue 4 is glutamic acid, amino acid residue 11 is asparagine, amino acid residue 14 is threonine, amino acid residue 23 is aspartic acid, amino acid residue 28 is glutamic acid, amino acid residue 41 is glutamic acid, amino acid residue 43 is glutamic acid, amino acid residue 100 is glutamic acid, amino acid residue 104 is threonine, amino acid residue 142 is aspartic acid, amino acid residue 152 is proline, amino acid residue 153 is glutamic acid, and amino acid residue 162 is glutamic acid, and wherein the SFP detector complements with an SFP tag to form a functional SFP.
An isolated polypeptide comprising a Split Fluorescent Protein (SFP) tag comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 4, wherein amino acid residue 2 is glutamic acid, amino acid 4 is isoleucine, and amino acid 17 is glutamic acid, and wherein SFP tag complements with an SFP detector to form a functional SFP.
An isolated polypeptide comprising a green fluorescent protein comprising:
- (i) an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 8, wherein amino acid residue 206 is glutamic acid, amino acid residue 208 is isoleucine, and amino acid residue 221 is glutamic acid;
- or
- (ii) an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
- 7, wherein amino acid residue 4 is glutamic acid, amino acid residue 11 is asparagine, amino acid residue 14 is threonine, amino acid residue 23 is aspartic acid, amino acid residue 28 is glutamic acid, amino acid residue 41 is glutamic acid, amino acid residue 43 is glutamic acid, amino acid residue 100 is glutamic acid, amino acid residue 104 is threonine, amino acid residue 142 is aspartic acid, amino acid residue 152 is proline, amino acid residue 153 is glutamic acid, amino acid residue 162 is glutamic acid, amino acid residue 206 is glutamic acid, amino acid residue 208 is isoleucine, and amino acid residue 221 is glutamic acid.
Dependencies are inferred from the claim text.