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US 12,630,811 B2Granted
Lactase enzymes with improved properties at acidic PH
AssigneeChr. Hansen A/S
InventorsHans Raj, Johannes Maarten Van Den Brink, Christian Gilleladen
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Abstract
The present invention relates to new improved peptide or dimeric peptides exhibiting betagalactosidase enzyme activity wherein the peptide has a pH optimum at acidic conditions.
Claims15
A process for producing a lactose-free or low-lactose acidified milk product, comprising:
- (a) acidifying a milk base to obtain an acidified milk product having a pH of 3.0 to 5.0, and
- (b) before, during, or after step (a), adding a bacterial peptide to the milk base or acidified milk product,
- wherein the bacterial peptide exhibits beta-galactosidase enzyme activity which has an activity optimum at a pH of below pH 6.7 and wherein the bacterial peptide is selected from peptides having the amino acid sequence of any one of SEQ ID NOs. 1, 2, 3 and 14, and
- wherein the acidified milk product has a lower lactose level than an acidified milk product produced by a process where no bacterial peptide is added, but is otherwise identical to the process as claimed.
1 more element
Dependencies are inferred from the claim text.