Method for biomaterial purification and kits thereof
AssigneeLife Technologies Corporation
InventorsKristopher E. Richardson, Daniel J. O'Neal, Jerald K. Rasmussen, Andrew W. Vail
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Abstract
Described herein is a method of purifying a target molecule from an aqueous biological composition, the method comprising: (a) contacting a cationic polymer and the aqueous biological composition to form a mixture comprising a bio-polymer complex and the target molecule in a liquid, wherein the bio-polymer complex has an average particle diameter of at least 45 micrometers, (b) adding the mixture to a filtering volume of a vessel, wherein the vessel comprises loosely packed staple fibers; (c) allowing the mixture to separate through the loosely packed staple fiber; and (d) collecting a filtrate comprising the target molecule.
Claims19
A method of purifying a target non-binding molecule from an aqueous biological composition comprising a binding species, the method comprising:
- (a) contacting a cationic polymer and the aqueous biological composition to form a mixture comprising a bio-polymer complex and the target non-binding molecule in a liquid, wherein the bio-polymer complex has an average particle diameter of at least 45 micrometers,
- (b) adding the mixture to a filtering volume of a vessel, wherein the vessel comprises loosely packed staple fibers;
- (c) allowing the mixture to separate through the loosely packed staple fibers;
- and
- (d) collecting a filtrate comprising the target non-binding molecule;
- wherein said loosely packed staple fibers are not cationically functionalized.
A kit comprising:
- (a) plurality of staple fibers wherein the staple fibers are loosely packed in a bed;
- and
- (b) a cationic polymer;
- wherein said staple fibers are not cationically functionalized.
Dependencies are inferred from the claim text.