Nucleic acid amplification
AssigneeHewlett-Packard Development Company, L.P.
InventorsViktor Shkolnikov, Alexander Govyadinov
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Abstract
Examples relate to techniques for performing a nucleic acid amplification reaction. The method includes generating a nucleic acid solution comprising a plurality of nucleic acid molecules, and combining the nucleic acid solution with a plurality of chamber particles. Each chamber particle includes a chamber for receiving the nucleic acid solution, wherein the chamber receives, at most, one of the plurality of nucleic acid molecules. Each chamber particle also includes reagents for causing a polymerase chain reaction within the chamber. The method further includes inducing nucleic acid amplification to generate an amplified nucleic acid, and performing a detection process to detect the presence of the amplified nucleic acid within the chamber.
Claims4
A method of performing a nucleic acid amplification reaction, comprising:
- generating a nucleic acid solution comprising a plurality of nucleic acid molecules;
- combining the nucleic acid solution with a plurality of chamber particles, each chamber particle comprising:
- a chamber for receiving the nucleic acid solution, wherein the chamber comprises a hollow space surrounded by walls forming a single opening of the chamber, and wherein the chamber receives, at most, one of the plurality of nucleic acid molecules through the single opening into the hollow space;
- and
- reagents for causing a polymerase chain reaction within the chamber,
- wherein the chamber particle comprises a magnetic material;
- moving the chamber particles from an aqueous phase to an oil phase via a magnetic field;
- inducing the nucleic acid amplification reaction to generate an amplified nucleic acid while the chamber particles are in the oil phase;
- and
- performing a detection process to detect the presence of the amplified nucleic acid within the chamber.
Dependencies are inferred from the claim text.