Apparatuses and methods for performing multiple omics analysis and processing analyte mixtures

A method of determining multiple omics of a single biological cell, the multiple omics including at least a first omics and a second omics, by performing in parallel multiple omics analyses on a plurality of droplets having cellular components originating from the single biological cell, the multiple omics analyses including at least a first omics analysis and a second omics analysis, the method comprising:

• isolating a single biological cell by providing a cell isolation droplet having only a single biological cell;
• providing a cell lysate droplet by lysing the isolated single biological cell in the cell isolation droplet;
• preparing the plurality of droplets including at least a first droplet and a second droplet having the cellular components from the lysed isolated single biological cell by dividing the cell lysate droplet;
• acquiring a first set of analytical data of the single biological cell by performing the first omics analysis on the first droplet using a first plurality of analytical techniques;
• acquiring a second set of analytical data of the single biological cell by performing the second omics analysis on the second droplet using a second plurality of analytical techniques, wherein the first plurality of analytical techniques is different from the second plurality of analytical techniques and the first set of analytical data is different from the second set of analytical data;
• and
• determining the first omics of the lysed isolated single biological cell by processing the first set of analytical data and determining the second omics of the lysed isolated single biological cell by processing the second set of analytical data.