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US 12,629,674 B2Granted
Rapid assessment device for radiation exposure
AssigneeVisca, LLC
InventorsGregory W. Auner, Michelle A. Brusatori, Changhe Huang
Life of this patent
Estimating the term…
Abstract
A cartridge includes a substrate including a polymerase chain reaction (PCR) zone. The PCR zone includes a first heating region, a second heating region spaced away from the first heating region and a detection region. A microchannel is formed in the substrate. The microchannel receives a fluid flowing therethrough, the microchannel passing through the first heating region and second heating region to thermally cycle the fluid. The microchannel passes through the detection region after the fluid has been thermally cycled.
Claims11
A cartridge comprising:
- a substrate including a polymerase chain reaction (PCR) zone including:
- a first heating region;
- a second heating region spaced away from the first heating region;
- a detection region, the detection region arranged between the first heating region and the second heating region;
- a microchannel formed in the substrate, the microchannel configured to receive a fluid flowing therethrough, the microchannel passing through the first heating region and second heating region to thermally cycle the fluid, and the microchannel passing through the detection region after the fluid has been thermally cycled;
- a cover having a first side and a second side opposite the first side, the second side of the cover attached to the substrate and enclosing the microchannel;
- a first heating element arranged on the first side of the cover in the first heating region;
- a second heating element arranged on the first side of the cover in the second heating region;
- and
- a blister formed on the first side of the cover, the blister containing a fluid and being fluidly coupled to the PCR zone, such that, when the blister is compressed, the fluid flows into the PCR zone;
- wherein the microchannel extends into a pre-PCR zone that preheats the fluid prior to the fluid entering the polymerase chain reaction (PCR) zone, and
- wherein the first heating region of the PCR zone extends to include an area of the substrate occupied by the pre-PCR zone.
9 more elements
A cartridge comprising:
- a substrate including a polymerase chain reaction (PCR) zone including:
- a first heating region;
- a second heating region spaced away from the first heating region;
- a detection region, the detection region arranged between the first heating region and the second heating region;
- a microchannel formed in the substrate, the microchannel configured to receive a fluid flowing therethrough, the microchannel passing through the first heating region and second heating region to thermally cycle the fluid, and the microchannel passing through the detection region after the fluid has been thermally cycled;
- a cover having a first side and a second side opposite the first side, the second side of the cover attached to the substrate and enclosing the microchannel;
- and
- a blister formed on the first side of the cover, the blister containing a fluid and being fluidly coupled to the PCR zone, such that, when the blister is compressed, the fluid flows into the PCR zone;
- wherein the microchannel extends into a pre-PCR zone that preheats the fluid prior to the fluid entering the polymerase chain reaction (PCR) zone, and
- wherein the first heating region of the PCR zone extends to include an area of the substrate occupied by the pre-PCR zone.
7 more elements
Dependencies are inferred from the claim text.