Method of analysis of polynucleotides by restricted access reversed phase chromatography
AssigneeWaters Technologies Corporation
InventorsMatthew A Lauber, Catalin Doneanu, Weibin Chen, Ying Qing Yu, Jennifer M. Nguyen, Fabrice Gritti, Martin Gilar
Life of this patent
Estimating the term…
Abstract
The present disclosure discusses a method of separating and/or purifying polynucleotides. The method includes injecting a sample into a chromatographic column that is packed with a porous sorbent having a pore size that substantially excludes the polynucleotides from the sorbent. This restricted access to the sorbent allows separation of large polynucleotides from each other and from smaller molecular weight impurities.
Claims30
A method of separating and/or purifying polynucleotides, comprising:
- injecting a sample comprising one or more polynucleotides into a chromatographic system, wherein the chromatographic system comprises a chromatographic column comprising a porous sorbent, wherein the average pore diameter of the porous sorbent is selected such that the polynucleotides are substantially excluded from the sorbent pores and has an average pore size of less than or equal to 300 Å;
- and
- flowing the sample through the chromatographic system under reversed phase chromatography conditions.
A method of separating and/or purifying polynucleotides, comprising:
- injecting a sample comprising one or more polynucleotides into a chromatographic system, wherein the chromatographic system comprises a chromatographic column comprising a porous sorbent, wherein the average pore diameter of the porous sorbent is selected such that the polynucleotides are substantially excluded from the sorbent pores and wherein the one or more polynucleotides have a pore accessibility in the porous sorbent of less than about 10%;
- and
- flowing the sample through the chromatographic system under reversed phase chromatography conditions.
A method of separating and/or purifying polynucleotides, comprising:
- injecting a sample comprising one or more polynucleotides into a chromatographic system, wherein the chromatographic system comprises a chromatographic column comprising a porous sorbent, wherein the average pore diameter of the porous sorbent is selected such that the polynucleotides are substantially excluded from the sorbent pores;
- heating the chromatographic column with a heater to a temperature sufficient to denature double stranded polynucleotides in the sample;
- and
- flowing the sample through the chromatographic system under reversed phase chromatography conditions.
Dependencies are inferred from the claim text.