Membrane sealing layer and spacer ring for viral clearance chromatography device

A chromatography device comprising:

• a housing having an inlet and an outlet;
• at least two layers of media disposed between the inlet and the outlet inside of the housing forming a media stack, with at least one of the layers comprising a functionalized layer;
• a spacer ring disposed between the two layers of media forming an air gap between them;
• a non-functionalized sealing layer disposed between the inlet and the outlet inside of the housing as the last and final layer of media in a media stack within the housing, and wherein a fluid is configured to pass from the inlet to the outlet through the media stack;
• and
• an edge of the sealing layer in contact with the housing;
• the edge configured to be compressed by the housing and forming a compressive seal between the sealing layer and the housing, and prevent the fluid from leaking to the outlet past the compressive seal.