Artificially synthesized sphingosine derivative lipoid monomer and use of same for delivering nucleic acid

A method for delivering a nucleic acid to a cell or a subject, comprising:

• (1) providing an organic solvent solution comprising a lipid composition;
• (2) providing an aqueous solution comprising the nucleic acid;
• (3) mixing the organic solvent solution of step (1) with the aqueous solution of step (2), and then heating at a temperature of 60° C. to 100° C. to obtain a lipid-nucleic acid mixture, and
• (4) contacting the lipid-nucleic acid mixture of step (3) with the cell or the subject so that the nucleic acid is delivered to the cell or the subject,
• wherein
• (i) the lipid composition consists of compound No.41, compound No.38 and one or more compounds of Formula (I), a stereoisomer or a pharmaceutical acceptable salt thereof;
• or
• (ii) the lipid composition consists of compound No.41, compound No.38, compound No.48 and one or more compounds of Formula (I), a stereoisomer or a pharmaceutical acceptable salt thereof;
• wherein compound No.41, compound No.38, and compound No.48 have the following structures respectively:
• wherein the compound of Formula (I) has a structure of:
• wherein:
• R1 is selected from C1-20 alkyl or C2-20 alkenyl, which is optionally substituted by one to three hydroxyl groups;
• R2 is hydrogen and R3 is hydroxyl;
• or
• R2 and R6 together for oxo (═O);
• R4 and R5 are independently selected from the group consisting of hydrogen, C1-20 alkyl, C1-30 alkylacyl and C2-30 alkenylacyl, said C1-30 alkylacyl and C2-30 alkenylacyl are optionally substituted by one to three groups selected from biotin, acyl or hydroxyl;
• R6 is selected from the group consisting of hydrogen, hydroxyl, phosphate ester group, —O-glycosyl, Ganglioside, and NH2—CH2—CH2—O—P(O)OH—.