Preparation of insoluble polysaccharides obtained from plant cell cultures in suspension for the treatment of clostridium difficile infections
AssigneeAbresearch Srl
InventorsRenzo Dal Monte, Andrea Carpi, Ignazio Castagliuolo, Paola Brun
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Abstract
An insoluble cell fraction obtained from plant cells cultured in a suspension composed entirely of natural molecules for the treatment of primary and recurrent Clostridium difficile infections is provided. A process for the preparation of the insoluble cell fraction is also provided.
Claims20
A method for preventing and/or treating Clostridium difficile infections, said method comprising administering to a subject in need thereof a pharmaceutically effective amount of an insoluble cell fraction obtained by a process comprising the steps of:
- a) obtaining a callus from plant cells,
- b) culturing cells obtained from said callus in a culture medium,
- c) collecting said cultured cells,
- d) removing the culture medium,
- e) resuspending biomass in osmotic water,
- f) homogenizing cells of said biomass thus obtaining a suspension, and
- g) microfiltering said suspension thus obtaining the insoluble cell fraction;
- wherein said plant cells are cells of a plant selected from the group consisting of:
- Lippia citriodora, Ajuga Reptans, Echinacea angustifoglia, Buddieja davidii, Ocimum basilicum, Campanula rapunculus, Melissa officinalis, Plantago lanceolata, Marrubbium vulgare, Ornithopus sativum, Glycyrrhiza grabra, Glycyrrhiza uralensis, Glycyrrhiza echinata, Petroselium crispum, Brassica oleracea, Eruca vescicaria, and Solanum lycopersicum.
A method comprising utilizing an insoluble cell fraction obtained by a process comprising the steps of:
- a) obtaining a callus from plant cells,
- b) culturing cells obtained from said callus in a culture medium,
- c) collecting said cultured cells,
- d) removing the culture medium,
- e) resuspending biomass in osmotic water,
- f) homogenizing cells of said biomass thus obtaining a suspension, and
- g) microfiltering said suspension thus obtaining the insoluble cell fraction;
- wherein said method comprises utilizing said insoluble cell fraction so as to inactivate Clostridium difficile toxin A and toxin B;
- wherein said plant cells are cells of a plant selected from the group consisting of:
- Lippia citriodora, Ajuga Reptans, Echinacea angustifoglia, Buddieja davidii, Ocimum basilicum, Campanula rapunculus, Melissa officinalis, Plantago lanceolata, Marrubbium vulgare, Ornithopus sativum, Glycyrrhiza grabra, Glycyrrhiza uralensis, Glycyrrhiza echinata, Petroselium crispum, Brassica oleracea, Eruca vescicaria, and Solanum lycopersicum.
Dependencies are inferred from the claim text.