US 12,629,401 B2Granted

Method for preparing ginseng extract enriched with rare ginsenosides and applications thereof

AssigneesChenland Nutritionals Inc., Qingdao Marine Energizing Biotechnology Development Co., Ltd.

InventorsShengcan Zou, Dongli Yin, Jinli Chen, Jinli Liu, Decui Yin, Haijun Zhang

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Abstract

A method for preparing ginseng extract rich in rare ginsenosides is disclosed. Ginseng roots or ginseng stems and leaves are extracted, enzymatically hydrolyzed and treated with high temperature and high pressure. The preparation method can quickly and effectively increase the proportion of rare ginsenosides in the ginseng extract. After in vitro antioxidant experiments and in vivo experiments with Hidradenitis elegans nematode, the extract and related complexes were proved to be effective in antioxidant, anti-oxidative stress, and improving age-related muscle decline and other dysfunctions. After genetic and metabolomic studies, the anti-aging mechanism was proved to be related to the expression of genes associated with nutrient sensing, stress-related pathways and redox processes, and showed innate immune response and redox process GO enrichment, proving its oxidative stress resistance.

Claims6

Dependent claims

A method for preparing ginseng extract rich in rare ginsenosides (GS), characterized in that it is prepared by the method of enzymatic hydrolysis and heat treatment, comprising:

(1) preparing ginseng stems and leaves extract dry powder or ginseng roots extract dry powder with water to form a substrate with a concentration of 10-20% (w/v), and adjusting a pH to 4.3-5.0;
(2) adding 2˜5 U/g β-glucosidase for enzymatic hydrolysis under 40˜60° C. for 3˜5 h;
after enzymatic hydrolysis;
heating for 1˜4 h at 115˜132° C. and 70˜200 kPa after the enzymatic hydrolysis;
(3) concentrating, under a temperature of 75˜85° C. and a concentration pressure of −0.05˜−0.09 Mpa, until a specific gravity is 1.1-1.2 g/L to obtain the concentrated solution;
the temperature of the concentrated solution during the measuring of the specific gravity is 25° C.;
(4) subjecting the concentrated solution obtained in the step (3) to centrifugal spray drying with following conditions:
inlet air temperature 135-160° C., outlet air temperature 80-100° C., centrifugal frequency 250-280 Hz, induced air frequency 50-60 Hz, and feed pump 10-40RPM.

5 more elements

Dependencies are inferred from the claim text.