Recombinant herpes simplex virus for multiple targeting and use thereof
AssigneeGencellmed Inc.
InventorsHeechung Kwon, Hyunjung Baek
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Abstract
Proposed are a recombinant herpes simplex virus for multiple targeting and the use thereof. Particularly, a recombinant HSV capable of multiple targeting through multiple expression of an adapter, which is a fused protein of a cancer-cell-targeting domain and an extracellular domain of HVEM, a recombinant HSV capable of multiple targeting by having a modified glycoprotein so as to enable retargeting, in addition to being capable of expressing the adapter that is the fused protein of the cancer-cell-targeting domain and the extracellular domain of HVEM, and the use of the virus for anti-inflammatory therapy are disclosed.
Claims17
A recombinant herpes simplex virus for multiple targeting comprising:
- (i) at least two expression cassettes, wherein each expression cassette is capable of expressing an adapter,
- wherein the adapter is a fused protein comprising a targeting domain that binds to a cancer cell target molecule and an extracellular domain of Herpesvirus Entry Mediator HveA (HVEM) that binds to a glycoprotein gD, and
- wherein the at least two expression cassettes are inserted into a genome of a herpes simplex virus without inhibiting propagation of the herpes simplex virus;
- (ii) a leader sequence attached to the N-terminus of the cancer cell target domain or the extracellular domain of HVEM,
- wherein the expression cassettes are inserted between UL3 and UL4 genes, between UL26 and UL27 genes, between UL48 and UL49 genes, between UL53 and UL54 genes, or between US1 and US2 genes in the genome of the virus;
- and
- (iii) another targeting domain, wherein the another targeting domain binds to a cancer cell target molecule,
- wherein the another targeting domain is inserted and fused into a position between amino acids 29 and 30 in a glycoprotein gH,
- wherein the glycoprotein gH has a sequence consisting of SEQ ID NO:
- 3, and
- wherein the cancer cell target molecule is an antigen or a receptor on a surface of a cancer cell that is expressed only in a cancer cell or is overexpressed in a cancer cell compared to a normal cell.
Dependencies are inferred from the claim text.