Chitosomes or chitosan-coated liposomes, use thereof to obtain cosmetic or pharmaceutical compositions and preparation method thereof

Nanometric liposome vesicles for cosmetic or pharmaceutical compositions obtained by a method comprising the steps of:

• a) forming of a liposomal suspension by:
• dissolving a chosen phospholipid and a chosen surfactant in ethanol to form a first suspension;
• and
• dissolving molecules of an active pharmaceutical or cosmetic ingredient in pyrogen-free water and optionally glycerin to form a second suspension,
• mixing both the first suspension and the second suspension for a specified time to form the liposomal suspension and
• adding a preservative solution to the liposomal suspension;
• b) forming chitosomes by adding a dispersion of chitosan in acetic acid and water to the liposomal suspension of step a);
• and
• c) treatment of the chitosomes with a polyanion to cross-link the chitosan
• the nanometric liposome vesicle comprising:
• i) low molecular weight fungal chitosan;
• ii) at least one phospholipid;
• iii) at least one active pharmaceutical or cosmetic ingredient;
• iv) liposome;
• and
• v) at least one cross-linking agent;
• wherein the at least one phospholipid forms a double layer encapsulating the at least one active pharmaceutical or cosmetic ingredient, the low molecular weight fungal chitosan arranged to cover the double layer to form the nanometric liposome vesicles and the at least one cross-linking agent between the liposome and the low molecular weight fungal chitosan to stabilize bonds therebetween.