Compositions comprising recombianant Bacillus cells and an insecticide
AssigneeBASF SE
InventorsDamian Curtis, Brian Thompson
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Abstract
The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one particular insecticide disclosed herein in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and/or reducing overall damage of plants and plant parts.
Claims14
A composition comprising:
- a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising:
- (i) an endoglucanase comprising an amino acid sequence with at least 90% sequence identity to SEQ ID NO:
- 107;
- and
- (ii) an exosporium targeting sequence, an exosporium protein, or an exosporium protein fragment comprising an exosporium targeting sequence comprising:
- a targeting sequence comprising amino acids 1-35 of SEQ ID NO:
- 1;
- a targeting sequence comprising amino acids 20-35 of SEQ ID NO:
- 1;
- a targeting sequence comprising amino acids 22-31 of SEQ ID NO:
- 1;
- a targeting sequence comprising amino acids 22-33 of SEQ ID NO:
- 1;
- a targeting sequence comprising amino acids 20-31 of SEQ ID NO:
- 1;
- a targeting sequence comprising SEQ ID NO:
- 1;
- or
- an exosporium protein comprising an amino acid sequence having at least 85% identity with SEQ ID NO:
- 2;
- and
- b) clothianidin
- in a synergistically effective amount,
- wherein the recombinant Bacillus cells are derived from Bacillus thuringiensis BT013A, wherein strain BT013A has been deposited under Agricultural Research Service Culture Collection Accession NRRL No. 50924.
A method of treating a plant, a plant part, or the locus surrounding the plant to enhance plant growth and/or promote plant health comprising the step of simultaneously or sequentially applying:
- a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising:
- (i) an endoglucanase comprising an amino acid sequence with at least 90% sequence identity to SEQ ID NO:
- 107;
- and
- (ii) an exosporium targeting sequence, an exosporium protein, or an exosporium protein fragment comprising an exosporium targeting sequence comprising:
- a targeting sequence comprising amino acids 1-35 of SEQ ID NO:
- 1;
- a targeting sequence comprising amino acids 20-35 of SEQ ID NO:
- 1;
- a targeting sequence comprising amino acids 22-31 of SEQ ID NO:
- 1;
- a targeting sequence comprising amino acids 22-33 of SEQ ID NO:
- 1;
- a targeting sequence comprising amino acids 20-31 of SEQ ID NO:
- 1;
- a targeting sequence comprising SEQ ID NO:
- 1;
- or
- an exosporium protein comprising an amino acid sequence having at least 85% identity with SEQ ID NO:
- 2;
- and
- b) clothianidin
- in a synergistically effective amount,
- wherein the recombinant Bacillus cells are derived from Bacillus thuringiensis BT013A, wherein strain BT013A has been deposited under Agricultural Research Service Culture Collection Accession NRRL No. 50924.
Dependencies are inferred from the claim text.