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US 12,628,750 B2Granted
Plant cell chromosome doubling by application of electromagnetic field
AssigneesPurdue Research Foundation, Syngenta Crop Protection AG
InventorsWilliam Paul Bullock, Rachel L. Egger, Allen L. Garner, Aginiprakash Dhanabal
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Abstract
Disclosed are methods for the doubling of a plant cell's chromosome by applying an electromagnetic field and methods for obtaining a doubled-haploid plant cell. In some embodiments, the plant cell is selected from the group comprising maize, rice, tomato, and tobacco. Also disclosed is an apparatus for applying an electromagnetic field to a plant cell.
Claims22
A method for chromosome doubling in a plant cell, the method comprising:
- a) applying an electromagnetic field (“EMF”) to a plant cell in vitro;
- and
- b) allowing the EMF to disrupt mitosis;
- wherein the plant cell replicates its chromosomes without undergoing cell division, thereby obtaining a plant cell having doubled chromosomes,
- wherein the EMF frequency is applied at a range of 100 kHz to 500 kHz, inclusive,
- wherein the EMF intensity is applied at a range of 0.5 V/cm to 100 V/cm, and
- wherein the EMF is applied at a duration selected from a group consisting of (i) at least a duration equivalent to prophase of mitosis for the plant cell;
- (ii) a duration equivalent to an entire mitosis cycle for the plant cell;
- and (iii) about 5 hours.
6 more elements
A method for producing a doubled-haploid plant cell, comprising:
- a) obtaining a haploid plant cell;
- b) subjecting the haploid plant cell to an electromagnetic field (“EMF”) in vitro;
- and
- c) allowing the EMF to disrupt mitosis in the haploid plant cell;
- whereby the haploid plant cell replicates its chromosomes yet fails to undergo cell division, thereby producing the doubled-haploid plant cell,
- wherein the EMF is an AC field with an applied electric field from 0.5 V/cm to 100 V/cm with a frequency from 100 kHz to 500 kHz, or
- wherein the EMF is an electric pulse with duration from 10 ns to 1 ms, an applied electric field from 1 V/cm to 300 kV/cm, rise- and fall-times from 0.5 ns to 5000 ns, and a repetition rate from 0.1 Hz to 100 Hz.
4 more elements
Dependencies are inferred from the claim text.