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US 12,625,142 B2Granted
Assay for measuring binding affinity for cardiolipin of biologically active compounds
AssigneeLatvian Institute of Organic Synthesis
InventorsPavels Arsenjans, Pavels Dimitrijevs
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Abstract
The present invention relates to a method for the evaluation of binding affinity of biologically active substances for cardiolipin based on acridinium salt utilization as a fluorescent probe.
Claims13
An assay method, comprising:
- incubating a solution comprising a compound of interest, a lipid vesicle containing cardiolipin, and 3,6-di(azetidin-1-yl)-10-(3-(trimethylsilyl)propyl)acridin-10-ium iodide (I)
- measuring fluorescence intensity at an excitation wavelength of 497 nm and at an emission wavelength of 529 nm, respectively.
A kit comprising:
- 3,6-di(azetidin-1-yl)-10-(3-(trimethylsilyl)propyl)acridin-10-ium iodide;
- a buffer;
- and
- a material containing cardiolipin.
1 more element
Dependencies are inferred from the claim text.